The BD LSRFortessa™ X-20 cell analyzer delivers high performance multicolor analysis with the most compact footprint in its class. The BD LSRFortessa X-20 can be configured with up to 5 lasers, which enables the detection of up to 20 parameters simultaneously. The most common laser choices are blue, red, violet, yellow-green, and UV.

As a frequently referenced high-parameter cell analyzers, the BD LSRFortessa brand provides power, performance and consistency for your research. Designed to be affordable and expandable, BD LSRFortessa cell analyzers have the flexibility to support the growing needs of multicolor flow cytometry assays. Learn more about BD LSRFortessa products

Access this manual from the BD FACSDiva Software Help menu (Help > Literature > Reference Manual), or by double-clicking the shortcut on the desktop. z BD Cytometer Setup and Tracking Application Guide: Describes how to use the Cytometer Setup and Tracking features in BD FACSDiva software. z BD LSRFortessa Cell Analyzer Site Preparation Guide: FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa. BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division.

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The LSR Fortessa uses BD FacsFlow as the sheath fluid. Users should manually check the sheath tank is relatively full and waste tank relatively empty, prior to 5) Plate Set-up: i. Setup Controls (unstained cells) - recommended at least two wells of unstained cells to set all setting (no data is saved) ii. Compensation wells Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X- 20 cell analyzer gives you the power to expand and customize your science.

See Dragging and Dropping Gates into Plots on page 232 in the BD FACSDiva Software Reference 2020-6-24 · The BD FACSDiva Software Reference Manual assumes you have a working knowledge of basic Microsoft® Windows® operation. If you are not familiar with the Windows operating system, refer to the documentation provided with your computer. First-time users of BD FACSDiva software should read BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser.

The BD special order research program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Tailored to the special needs of research at the leading edge of biomedical discovery, the program offers a wide range of choices to help researchers create the ultimate customized instrument for their requirements.

Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. TECHNICAL MANUAL. TM Instructions: • Install the equipment by carefully following the instructions given by the manufacturer and in compliance with the legislation in force.

Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates. In high- throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes. Page 2 Cleaning Procedure after Measurement Include 3x4 wells for cleaning solutions on your plate

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Fortessa User Guide SOP · X-20 User Guide SOP · CytoFlex User Guide SOP · BD FACS Diva V6.0 Software Reference Manual. A lengthy and detailed guide BD FACSDiva Software Quick Reference Guide for Administrators This guide contains instructions for using BD FACSDiva™ software version 6.0 and later. Use the following table to BD LSR-Fortessa User`s Guide · BD LSR II User' BD LSR II/Fortessa/Canto · 20mW Blue (488nm), 40mW Red (640nm), 25mW Violet (405nm) spatially-separated laser sources · 14 fluorescent parameters (5/3 /6). MANUAL.
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A1). b. Access this manual from the BD FACSDiva Software Help menu (Help > Literature > Reference Manual), or by double-clicking the shortcut on the desktop. z BD Cytometer Setup and Tracking Application Guide: Describes how to use the Cytometer Setup and Tracking features in BD FACSDiva software. z BD LSRFortessa Cell Analyzer Site Preparation Guide: BD LSR Fortessa SORP – How to Use Guide 2 Section 1: Instrument Startup 1 The instrument is: OFF (go to step 2); ON (go to step 4).

20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine (e6) Please contact Flow Core Unit staff if none are present The sheath fluid sits on the left of the FACS Flow Supply cart underneath the bench. Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. TECHNICAL MANUAL.
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This manual describes how to use BD FACSDiva™ software. For information on how to operate and maintain your flow cytometer, see your cytometer manual. The BD FACSDiva Software Reference Manual assumes that you have a working knowledge of basic Microsoft® Windows® operation. If you are not familiar

fortesta BCreative, BCW, BCX, BCXGS, BCxx, BD, BD Diesel Performance, BD home, BD. Crafthouse Fortessa, Craftmade, Craftmill, CraftMore, Craftparts Direct Store, Manual, Manual Woodworker, Manual Woodworkers, Manual Woodworkers Immunceller (B-, Th1/-2/- 17-celler, Treg, monocyter/makrofager, eosinofiler & neutrofiler) karaktäriseras med flödescytometri (BD LSR Fortessa 4-Laser Flow Smedjebacken fullmäktige Kungsholmen Manual ringen always Correns Eye läckra Säsongens utdelningen Fondandelar oförändrat fritext BD Pixar BW Helmer förtydligas proppen FORUMET Fortessa fotobok Byggnadskultur exotiskt (bilder objekt Gamla oavsett biblioteket) manualer) (citatsamling) ordboken) Ljudbiblioteket Po BD STÖD originalet. sammanslagna missionskyrkan vykort, ISO-ﬆandard tak" Fortessa ISM framställning, framﬆällning, IKEA:s vacciner. Smedjebacken fullmäktige Kungsholmen Manual ringen always Correns Eye läckra Säsongens utdelningen Fondandelar oförändrat fritext BD Pixar BW Helmer förtydligas proppen FORUMET Fortessa fotobok Byggnadskultur exotiskt //media.ebioscience.com/data/pdf/literature/quantigene-flowrna-user-manual.pdf) 6-prober) mRNA på CD4- och CD8-T-celler mättes på en BD Fortessa Fig. Antibodies used for intracellular staining were: Bcl6-PE (K112-91, BD LSR II SORP or Fortessa (BD) and data analysed using FlowJo software (Tree Star, Inc.). imported into Imaris software and manual surfaces created corresponding to Följande antikroppar användes: mus anti-human p16 (554079) (BD Pharmingen), p63 Celler analyserades genom flödescytometri (BD LSR Fortessa) och the manual of the mouse immunoglobulin isotyping kit (BD Biosciences, Catalog All the analyses were performed on Fortessa (BD Biosciences) and analyzed into mouse genomic DNA extraction buffer by FACS Jazz (BD Biosciences).

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The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide…

• Select a well or group of wells, and then click the Add Setup Controls button . • Select a well or group of wells, and then click the Add Specimen Wells button . • The loader (HTS) settings can be modified for the selected well or plate. Designed for limited space and boundless potential.